Abstract

To provide markers to identify chromosomes in the genome of octopods, chromosomes of three octopus species were subjected to NOR/C-banding. In addition, we examined their genome size (Cvalue) to submit it to the Animal Genome Size Database. Silver staining revealed that the number of Ag-nucleoli was 2 (Octopusminor(Sasaki, 1920)), 2 (Amphioctopusfangsiao(d’Orbigny, 1839)) and 1 (CistopuschinensisZheng et al., 2012), respectively, and the number of Ag-nucleoli visible was the same as that of Ag-NORs on metaphase plates in the same species. In all analyzed metaphases, Ag-NORs were mainly located terminally on the long arms of chromosomes 3 (3rd) ofO.minorand on the short arms of chromosomes 4 (4th) ofA.fangsiao, whereas only one of the chromosomes 23 (23rd) was found Ag-NORs ofC.chinensis. C-bands were localized predominantly in the centromeric regions of chromosomes in the three species, while other conspicuous stable C-bands were observed in terminal regions, including the Ag-NORs. That means these three chromosome pairs (3rd, 4thand 23rd) could be considered species-specific cytogenetic markers. The meanCvalues ofO.minor,A.fangsiaoandC.chinensiswere 7.81±0.39 pg (0.070 pg per unit length), 8.31±0.18 pg (0.068 pg per unit length) and 5.29±0.10 pg (0.038 pg per unit length), respectively, and results showed thatCvalues of the three species were not proportional to the relative length of the chromosomes. These cytogenetic characteristics will provide more theoretical foundation for further researches on chromosome evolution in octopods.

Addeddate

2025-03-16 14:20:29

Bhl_virtual_titleid

210879

Bhl_virtual_volume

v.12:no.3 (2018)

Call number

10_3897_CompCytogen_v12i3_25462

Call-number

10_3897_CompCytogen_v12i3_25462

Foldoutcount

0

Genre

article

Identifier

cytogeneticstud12wang

Identifier-ark

ark:/13960/s2g8n1v8x42

Identifier-bib

10_3897_CompCytogen_v12i3_25462

Identifier-doi

10.3897/CompCytogen.v12i3.25462

Issue

3

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

Ocr_detected_lang_conf

1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

1.0000

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

67

Page_number_module_version

1.0.5

Page_range

373-386

Pages

14

Pdf_degraded

invalid-jp2-headers

Pdf_module_version

0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

Comparative Cytogenetics 12(3)

Year

2018

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